{"id":107,"date":"2021-09-22T18:15:48","date_gmt":"2021-09-22T16:15:48","guid":{"rendered":"http:\/\/yb-isn.fr\/2021\/spc\/?p=107"},"modified":"2021-12-03T10:24:32","modified_gmt":"2021-12-03T09:24:32","slug":"spectrophotometrie","status":"publish","type":"post","link":"https:\/\/yb-isn.fr\/2021\/spc\/?p=107","title":{"rendered":"Spectrophotom\u00e9trie (rappels)"},"content":{"rendered":"<p><!--more--><\/p>\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/yb-isn.fr\/ts42019\/wp-content\/uploads\/2019\/11\/spctrale-1024x433.png\" alt=\"\" class=\"wp-image-257\"\/><\/figure>\n\n\n\n<p class=\"has-very-light-gray-background-color has-background\">1)<strong>spectres UV_Visible<\/strong><\/p>\n\n\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-273 alignleft\" src=\"http:\/\/yb-isn.fr\/ts42019\/wp-content\/uploads\/2019\/11\/courbesAT-1-245x300.png\" alt=\"\" width=\"245\" height=\"300\"><\/p>\n<p><\/p>\n<p>La spectroscopie UV-Visible est l\u2019\u00e9tude de l\u2019absorption de certaines radiations \u00e9lectromagn\u00e9tiques (200nm \u00e0 1000nm) par un \u00e9chantillon de mati\u00e8re.<\/p>\n<p><\/p>\n<p>L\u2019appareil (spectrophotom\u00e8tre) &nbsp;enregistre I et I<sub>0<\/sub> pour diff\u00e9rentes valeurs de l puis calcule l\u2019absorbance A ou la transmittance T.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-258  aligncenter\" src=\"http:\/\/yb-isn.fr\/ts42019\/wp-content\/uploads\/2019\/11\/AetT-150x150.png\" alt=\"\" width=\"128\" height=\"128\"><\/p>\n<p><\/p>\n<p>Le spectre UV-Visible correspond au trac\u00e9 de A ou T en fonction de la longueur d&rsquo;onde .<\/p>\n<p><\/p>\n\n\n<p class=\"has-very-light-gray-background-color has-background\">2)<strong>spectrophotom\u00e9trie<\/strong><\/p>\n\n\n\n<p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\nL\u2019absorbance\nA d\u2019une solution \u00e0 une longueur d\u2019onde donn\u00e9e v\u00e9rifie la loi de Beer Lambert si\nA est proportionnel \u00e0 la concentration du solut\u00e9 qui absorbe. C\u2019est souvent le\ncas aux faibles concentrations.\n\n\n\n<\/p>\n\n\n\n<figure><iframe loading=\"lazy\" src=\"https:\/\/phet.colorado.edu\/sims\/html\/beers-law-lab\/latest\/beers-law-lab_en.html\" allowfullscreen=\"\" width=\"400\" height=\"300\"><\/iframe><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/yb-isn.fr\/ts42019\/wp-content\/uploads\/2019\/11\/beerlambert.png\" alt=\"\" class=\"wp-image-260\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/yb-isn.fr\/ts42019\/wp-content\/uploads\/2019\/11\/beerlambert1.png\" alt=\"\" class=\"wp-image-261\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/yb-isn.fr\/ts42019\/wp-content\/uploads\/2019\/11\/beerlambert2.png\" alt=\"\" class=\"wp-image-262\"\/><\/figure>\n\n\n\n<p class=\"has-very-light-gray-background-color has-background\">3)<strong>spectres et couleurs<\/strong><\/p>\n\n\n\n<figure><iframe loading=\"lazy\" src=\"https:\/\/phet.colorado.edu\/sims\/html\/color-vision\/latest\/color-vision_fr.html\" allowfullscreen=\"\" width=\"400\" height=\"300\"><\/iframe><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/yb-isn.fr\/ts42019\/wp-content\/uploads\/2019\/11\/couleurper\u00e7ue.png\" alt=\"\" class=\"wp-image-264\"\/><\/figure>\n\n\n\n<p><strong>La couleur\nest li\u00e9e \u00e0 un grand nombre de doubles liaisons conjugu\u00e9es. Certains atomes ou\ngroupes d\u2019atomes (auxochromes peuvent influencer la couleur).<\/strong><strong>Exemple&nbsp;: colorant alimentaire bleu patent\u00e9.<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/yb-isn.fr\/ts42019\/wp-content\/uploads\/2019\/11\/auxochrome.png\" alt=\"\" class=\"wp-image-267\"\/><\/figure>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":111,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-107","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cours"],"_links":{"self":[{"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=\/wp\/v2\/posts\/107"}],"collection":[{"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=107"}],"version-history":[{"count":3,"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=\/wp\/v2\/posts\/107\/revisions"}],"predecessor-version":[{"id":146,"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=\/wp\/v2\/posts\/107\/revisions\/146"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=\/wp\/v2\/media\/111"}],"wp:attachment":[{"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=107"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=107"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/yb-isn.fr\/2021\/spc\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=107"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}